Centrifugal Filter
RevoSeizer Centrifugal Filter device can quickly and effectively concentrate and purify biological samples, and currently offers specifications of 0.5ml, 4ml, and 15ml. The unique vertical design and maximum filtration area provide fast sample processing and high sample recovery rate (typically greater than 90% of dilute starting solution), while maintaining a gentle concentration environment to preserve protein activity and conformation. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The wide selection of ultrafiltration molecular weight cut-off (MWCO) devices incorporate PES membrane, which is very low in protein and nucleic acid binding.
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| Specs | Product | Optional Parameters | Availability | Operate |
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Related Documents
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| MS RevoSeizer Centrifugal Filter Datasheet | MS RevoSeizer Centrifugal Filter Quick Usage Guide |
Features
- Precise Retention, low protein adsorption, high recovery rate >90%
- Dual-Sided Vertical Membrane Design. Prevents membrane clogging, ultra-low holdup volume, high centrifugal efficiency
- Excellent Biocompatibility& Safety. Minimal extractables, USP87 certified
- Anti-Dry Filtration Locking Design.Prevents over-centrifugation to ensures stable and reliable experimentalresults
- Diverse Membrane Options
- OEM customization
Applications
The filtration process is carried out by depth filter sheets, which are available in a range of porosities, from coarse to fine, and even germ-reducing filtration.
Ultra-filtration centrifugal filter:
- Concentrate and desalt proteins, nucleic acids
- Buffer exchange or desalting of chromatographi components
- Harvest bio-molecules from culture media
- Virus concerntration or isolation
- Coarse separation of bio-molecule mixtures
- Removal of debris and particles from cell lysates
Micro-filtration centrifugal filter:
- Isolate DNA from agarose gel
- Separation of proteins, oligonucleotides and RNA from polyacrylamide gels
- Sample clarification before HPLC analysis
- Media removal of cells
- Filter biological samples
- Collect and clean processed particles or beads
- Fill chromatographic media for analytical procedures or process development
Technical Parameters
| Product Cat | 0.5ml | 4ml | 15ml |
| Filter | PC(Polycarbonate) | SBC(Styrene buta diene copolymer) | |
| Membrane | Polyethersulfone | ||
| Filtrate Tube | Polypropylene | ||
| Filtrate Cap and Liner | Polypropylene | ||
| Effective Filter Area | 0.9cm² | 3.5cm² | 7.2cm² |
| Filters in Test Tubes (Capped) |
Overall lengt(concentrated mode:filter inserted into outer tube):50.3mmOverall lengt(reverse centrifugation: |
Overall length 122mm Diameter 17mm |
Overall length 117mm Diameter31mm |
| Filter | Length:29.2 mm Diameter:9.4 mm | Length:67.5mm Diameter:14.5mm | Length:75mmDiameter:28mm |
| Retention Volume | ≤5μl | ≤20μl | ≤30μl |
| Locking Volume | 10-20μl | 50-100μl | 300μl |
| Operating Temperature | 0-40℃ | ||
| pH Range | 1-14 | ||
| Maximum Centrifugal Force(Ultra Filtration) |
14000x g | Swing bucket rotor4000x g | Swing bucket rotor 4000x g (100KD 3000x g) |
| Fixed angle rotor 6000x g | Fixed angle rotor5000xg (100KD 3000x g) |
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| Maximum Centrifugal Force(Micro Filtration) |
/ | Microfiltration:10000x g | Microfiltration:6000x g |
| Centrifuge Type | Centrifuge standard 1.5ml conical tubes | Centrifuge standard 15 ml conical tubes |
Centrifuge standard 50 ml conical tubes |
| Sterilization | Non-sterile,can be disinfected and sterilized by filtering 70% ethanol through the equipment. | ||
FAQ